In a 2014 mouse elevated-plus-maze study, an ethanolic extract of Convolvulus pluricaulis given orally at 500 mg/kg produced a mean open-arm time of 84.0 ± 12.5 seconds, compared with 23.4 ± 5.3 seconds in untreated controls. Diazepam at 1 mg/kg produced 156.6 ± 32.8 seconds. These results support an anxiolytic-like effect in one animal model, but they do not show that the extract has “54% of diazepam’s potency.” Potency cannot be calculated by directly comparing one behavioural endpoint at dissimilar doses, and the experiment did not establish clinical efficacy in people.

This captures the present state of Shankhpushpi research: a credible preclinical signal and a modern pharmacopoeial identity, but major uncertainty about species, extract composition, mechanism, human dosing, interactions, and effectiveness for diagnosed anxiety disorders.

The Identity Problem: Which Plant Is Shankhpushpi?

The first research question is botanical, not pharmacological. “Shankhpushpi” has been applied to more than one plant in Indian practice and commerce. A comparative pharmacognostic investigation published in 2010 documented four plants traditionally traded or used under the name:

  • Convolvulus pluricaulis Choisy (Convolvulaceae)
  • Evolvulus alsinoides L. (Convolvulaceae)
  • Clitoria ternatea L. (Fabaceae)
  • Canscora decussata (Roxb.) Schult. (Gentianaceae)

These names are not interchangeable experimental labels. The 2010 study compared morphology, microscopy, physicochemical constants, and chromatographic fingerprints to support authentication. A paper or product that says only “Shankhpushpi” may therefore be impossible to reproduce reliably.

The Ayurvedic Pharmacopoeia of India, Part I, Volume II, defines Shankhapushpi as the dried whole plant of Convolvulus pluricaulis Choisy. Its monograph describes a prostrate or sub-erect, spreading, hairy perennial herb with a woody rootstock. The same monograph notes that Clitoria ternatea and Evolvulus alsinoides are used as Shankhapushpi in certain parts of India. Thus, the pharmacopoeia supplies a reference identity while also acknowledging regional substitution.

A 2016 study developed an internal-transcribed-spacer multiplex PCR assay to distinguish Convolvulus prostratus—the name used by those authors for the plant also cited as C. pluricaulis—from E. alsinoides. It generated species-specific products of 200 and 596 base pairs and detected 10% admixture. Molecular testing is useful, but trials still need documented sourcing, voucher specimens, and chemical standardization.

Classical and Pharmacopoeial Profile

In the Charaka Samhita tradition, Shankhapushpi is grouped with Mandukaparni, Yashtimadhu, and Guduchi in medhya rasayana practice. “Medhya” concerns intellect, memory, and mental faculties within Ayurveda; it is not automatically a claim to treat generalized anxiety disorder, dementia, or another modern diagnosis.

The official pharmacopoeial monograph gives the following attributes for Convolvulus pluricaulis. These are the API entries and should not be replaced with properties copied from a different regional substitute or later secondary source:

Ayurvedic category API description
Rasa Tikta, Katu, Kashaya
Guna Snigdha, Pichchila
Virya Shita
Vipaka Madhura
Karma Tridoshahara, Rasayana, Medhya, Balya, Mohanashaka, Ayushya

The API lists manasaroga and apasmara among therapeutic uses and gives 3–8 g of powder as the monograph dose. These categories are not exact synonyms for contemporary anxiety disorders or epilepsy and require qualified clinical interpretation.

What the Anxiety Studies Actually Show

The evidence most often cited for anxiety consists mainly of rodent experiments. These studies can identify a behavioural signal and guide mechanism research, but they cannot determine whether an authenticated product is effective, safe, or appropriately dosed for a person with an anxiety disorder.

Study Material and design Verified finding Main limitation
Nahata and colleagues, 2009 Rodent experiments using ethyl-acetate fractions of E. alsinoides and C. pluricaulis Both materials produced anxiolytic-like effects in behavioural tests Animal data from selected fractions; not a human trial
Malik and colleagues, 2011 Extracts from three commonly used botanical sources of Shankhpushpi Nootropic, anxiolytic-like, and CNS-depressant activities varied by plant source and dose Source-dependent results prevent a single conclusion for every “Shankhpushpi” product
Siddiqui and colleagues, 2014 Mice given aqueous, ethanolic, or chloroform extracts of C. pluricaulis at 500 mg/kg All three extracts increased open-arm time; ethanolic extract reached 84.0 seconds versus 23.4 seconds in controls Single acute animal experiment with a high extract dose
Heba and colleagues, 2017 Mouse model of ethanol intake and withdrawal Shankhpushpi reduced withdrawal-associated anxiety-like behaviour and altered cortico-hippocampal GABA findings A model of alcohol withdrawal, not ordinary clinical anxiety

Open-arm time is a behavioural proxy, not a direct measure of calmness, receptor occupancy, or therapeutic equivalence. The extract also lacked a quantified marker specification. Because different botanical sources produce different CNS profiles, future trials should report the Latin binomial, plant part, solvent, extraction ratio, voucher specimen, contaminant testing, fingerprint, and marker range.

GABAergic Activity: A Plausible Pathway, Not a Settled Human Mechanism

The 2014 paper proposed possible involvement of the GABA-benzodiazepine system, but it did not measure receptor binding, chloride flux, or human GABA-A activity. Claims of proved benzodiazepine-site agonism or benefit without tolerance go beyond its evidence.

The 2017 ethanol-withdrawal experiment provides more specific preclinical support. In that mouse model, the anti-anxiety-like effect was blocked by a GABA-A antagonist, and chronic administration was associated with increased cortico-hippocampal GABA. This supports GABA-A involvement under the conditions of that experiment. It does not establish GABA-A as the sole mechanism of every preparation or predict the size of an effect in human anxiety.

Reviews report alkaloids, coumarins, flavonoids, and phytosterols in C. pluricaulis, including shankhpushpine, convolamine, scopoletin, kaempferol, and beta-sitosterol. Presence does not establish mechanism, and content varies with identity and extraction. The API gives only the broad constituent entry “alkaloid,” not a clinical standard for anxiety.

Mechanistic caution: A 2022 network-pharmacology paper predicted multiple targets and pathways relevant mainly to dementia research. Computational target mapping is hypothesis-generating. It cannot confirm that a compound reaches the human brain at an active concentration, binds the predicted target in vivo, or improves a clinical outcome.

Cognition and Memory: Promising Preclinical Data, Limited Clinical Proof

The cognition literature remains dominated by laboratory models. Rodent studies report effects after scopolamine- or aluminium-associated impairment. In one aluminium-neurotoxicity model, C. pluricaulis affected muscarinic M1 receptor, choline acetyltransferase, and NGF-TrkA expression. These findings do not establish prevention or treatment of human dementia.

Another rodent study used piracetam as a reference in learning and memory tests. A reference group does not establish clinical equivalence, and animal performance cannot be turned into a prescribing comparison.

Human evidence is modest. A 2015 comparative study enrolled 102 healthy young volunteers and evaluated four 500 mg Shankhapushpi tablets twice daily after food with milk for two months against a programme involving yoga and Satvavajaya Chikitsa. Some memory measures improved, but the herbal intervention was not tested against an inert placebo under a fully blinded design, and the participants did not have anxiety disorders or dementia. The study therefore offers preliminary information about cognitive testing, not proof of treatment for a psychiatric or neurodegenerative condition.

An Alzheimer’s Drug Discovery Foundation evidence review likewise concluded that well-controlled human data are lacking. Comparative trials have not established superior safety or efficacy over prescription drugs.

Dose and Preparation: What Can Be Stated Reliably

Mouse doses should not be converted into self-treatment doses by simple body-surface-area arithmetic. Such calculations do not account for extract ratio, constituent exposure, metabolism, product quality, or indication.

Preparation or context Verified amount How to interpret it
API whole-plant powder 3–8 g Pharmacopoeial monograph dose for the authenticated crude drug; not evidence of efficacy for generalized anxiety disorder
Tablet used in the 2015 volunteer study Four 500 mg tablets twice daily for 60 days A study-specific regimen in healthy young adults; not a universal clinical dose
Commercial syrups, capsules, and extracts No single evidence-based equivalent dose Concentration, species, plant part, extraction ratio, sugar content, and marker compounds may differ

A useful label states the botanical name, plant part, crude-drug equivalent, solvent and ratio, and batch testing. “Shankhpushpi 500 mg” is incomplete when products may contain powder, concentrated extract, or another species.

Safety and Herb–Drug Interactions

Human safety information is insufficient for confident claims about long-term use or combinations with psychiatric and neurological medicines. Traditional use cannot replace adverse-event monitoring and interaction studies.

  • Phenytoin: A 1992 report described two patients who lost seizure control while using a Shankhapushpi preparation with phenytoin. Repeated administration in rats reduced phenytoin concentration and anticonvulsant activity. Although botanical authentication was inadequate by current standards, patients should not combine them without the prescribing clinician.
  • Benzodiazepines, sedating antihistamines, alcohol, and other CNS depressants: Some extracts show CNS-depressant or sleep-potentiating activity in animals. Additive drowsiness is a reasonable precaution, although human risk is unquantified.
  • Antihypertensive treatment: Limited summaries note possible blood-pressure-lowering effects. A person with low blood pressure or prescribed antihypertensives should seek clinical advice and monitoring rather than assuming the combination is harmless.
  • SSRIs, SNRIs, and other antidepressants: Computational literature does not establish a clinical serotonin-syndrome interaction. Combination safety is inadequately studied.
  • Pregnancy, breastfeeding, children, liver or kidney disease: Adequate controlled safety data are lacking. Unsupervised medicinal use should be avoided in these groups unless an appropriately qualified clinician has assessed the individual situation.

Safety advice: Do not stop or replace prescribed anxiety, seizure, sleep, or blood-pressure treatment with Shankhpushpi. Discuss the exact product with the prescribing healthcare provider and a qualified Ayurvedic practitioner, especially when symptoms are severe or medicines are already used.

Research Gaps That Should Shape the Next Trial

The evidence does not show that Shankhpushpi “works like a benzodiazepine.” It shows that authenticated C. pluricaulis deserves better-designed human research addressing the following gaps.

  1. Diagnosed anxiety populations: PubMed-indexed literature does not provide an adequately powered, double-blind, placebo-controlled trial of authenticated C. pluricaulis for a defined anxiety disorder.
  2. Botanical authentication: Trials need a voucher specimen and molecular or validated pharmacognostic confirmation, not only the common name Shankhpushpi.
  3. Chemical standardization: Extract ratio, solvent, fingerprint, marker range, contaminants, pesticides, microbes, and heavy metals should be reported batch by batch.
  4. Pharmacokinetics: Human absorption, active metabolites, brain exposure, half-life, and dose proportionality have not been established for a clinically standardized preparation.
  5. Safety and interactions: Studies should prospectively monitor sedation, cognition, blood pressure, liver and kidney indices, withdrawal effects, and interactions with commonly prescribed medicines.
  6. Clinically meaningful outcomes: A future trial should use a preregistered primary anxiety scale, functional outcomes, adverse-event reporting, and follow-up after discontinuation rather than relying only on laboratory memory tests or subjective impressions.

Where the Evidence Leaves Us

Shankhpushpi has a genuine Ayurvedic context, an API monograph for Convolvulus pluricaulis, and anxiolytic-like and cognitive signals in animal models. That justifies investigation, not a percentage comparison with diazepam, confirmed GABA-A agonism in people, or replacement of evidence-based anxiety care.

A defensible next step is a preregistered, randomized, double-blind, placebo-controlled study using authenticated, chemically characterized C. pluricaulis, preceded by dose-finding and safety work. Until then, species verification, professional supervision, medication review, and honest uncertainty are more appropriate than promotional certainty.

References

  1. Openneurologyjournal (openneurologyjournal.com)
  2. Neuropharmacological Profile of Extracts of Aerial Parts of Convolvulus pluricaulis Choisy in Mice Model (2014), PubMed
  3. Comparative pharmacognostical investigation on four ethanobotanicals traditionally used as Shankhpushpi in India (2010), PubMed
  4. Comparative pharmacognostical investigation on four ethanobotanicals traditionally used as Shankhpushpi in India (2010), PubMed Central
  5. Ayurvedic Pharmacopoeia of India
  6. Nootropic herbs (Medhya Rasayana) in Ayurveda: An update (2012), PubMed Central
  7. Internal transcribed spacer guided multiplex PCR for species identification of Convolvulus prostratus and Evolvulus alsinoides (2016), PubMed Central
  8. Tandfonline (tandfonline.com)
  9. Nootropic, anxiolytic and CNS-depressant studies on different plant sources of shankhpushpi (2011), PubMed
  10. Effect of Shankhpushpi on Alcohol Addiction in Mice (2017), PubMed Central
  11. Frontiersin (frontiersin.org)
  12. Protective Mechanisms of Nootropic Herb Shankhpushpi (Convolvulus pluricaulis) against Dementia: Network Pharmacology and Computational Approach (2022), PubMed Central
  13. In vivo investigation of the neuroprotective property of Convolvulus pluricaulis in scopolamine-induced cognitive impairments in Wistar rats (2011), PubMed Central
  14. Neuroprotective role of Convolvulus pluricaulis on aluminium induced neurotoxicity in rat brain (2009), PubMed
  15. Nootropic efficacy of Satvavajaya Chikitsa and Ayurvedic drug therapy: A comparative clinical exposition (2015), PubMed
  16. Alzdiscovery (alzdiscovery.org)
  17. Analysis of a clinically important interaction between phenytoin and Shankhapushpi, an Ayurvedic preparation (1992), PubMed

Nothing in this article diagnoses or treats a medical condition. Use it as educational information and consult a qualified Ayurvedic practitioner or physician before starting herbs, supplements, detoxes, or therapeutic protocols, especially if pregnant, managing a condition, or taking medication.